{"id":"scrna-seq","type":"method","name_en":"Single-cell RNA sequencing (scRNA-seq)","name_zh":"单细胞 RNA 测序（scRNA-seq）","aliases":["scRNA-seq","scRNA","single-cell RNA-seq","single-cell transcriptomics"],"description":{"en":"Assay underlying most cell-state studies and atlases in the library, for example the Neftel study of 28 tumors and the GBmap atlas of more than one million cells.","zh":"文献库中多数细胞状态研究与图谱的基础技术，例如 Neftel 对 28 例肿瘤的研究与逾百万细胞的 GBmap 图谱。"},"externalIds":{},"graphNames":["single-cell RNA-seq"],"mentions":{"items":["VC0008","VC0011","VC0030","VC0057","VC0059","VC0063","VC0064","VC0065","VC0068","VC0072","VC0079","VC0080","VC0082","VC0083","VC0087","VC0132"],"topics":["cell-state-mes-like","dataset-gbm-single-cell-spatial"]},"evidenceRefs":["VC0059","VC0060","VC0063","VC0064"],"reviewStatus":"draft","version":"0.1.0","page_url":"https://gliostate.com/entities/scrna-seq/","page_url_zh":"https://gliostate.com/zh/entities/scrna-seq/","api_url":"https://gliostate.com/api/entities/scrna-seq.json","external_links":[],"source_file":"https://gliostate.com/data/entities/scrna-seq.json","evidence_items":[{"id":"VC0059","title":"An Integrative Model of Cellular States, Plasticity, and Genetics for Glioblastoma.","year":"2019","page_url":"https://gliostate.com/library/VC0059/","api_url":"https://gliostate.com/api/items/VC0059.json"},{"id":"VC0060","title":"Charting the single-cell and spatial landscape of IDH-wild-type glioblastoma with GBmap.","year":"2025","page_url":"https://gliostate.com/library/VC0060/","api_url":"https://gliostate.com/api/items/VC0060.json"},{"id":"VC0063","title":"Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq.","year":"2017","page_url":"https://gliostate.com/library/VC0063/","api_url":"https://gliostate.com/api/items/VC0063.json"},{"id":"VC0064","title":"Developmental and oncogenic programs in H3K27M gliomas dissected by single-cell RNA-seq.","year":"2018","page_url":"https://gliostate.com/library/VC0064/","api_url":"https://gliostate.com/api/items/VC0064.json"}],"mentioned_in_items":[{"id":"VC0008","title":"scGPT: toward building a foundation model for single-cell multi-omics using generative AI.","year":"2024","page_url":"https://gliostate.com/library/VC0008/","api_url":"https://gliostate.com/api/items/VC0008.json"},{"id":"VC0011","title":"Large-scale foundation model on single-cell transcriptomics.","year":"2024","page_url":"https://gliostate.com/library/VC0011/","api_url":"https://gliostate.com/api/items/VC0011.json"},{"id":"VC0030","title":"scBERT as a Large-scale Pretrained Deep Language Model for Cell Type Annotation of Single-cell RNA-seq Data","year":"2021","page_url":"https://gliostate.com/library/VC0030/","api_url":"https://gliostate.com/api/items/VC0030.json"},{"id":"VC0057","title":"Assessing scale and predictive diversity in models for single-cell transcriptomics based on Geneformer.","year":"2026","page_url":"https://gliostate.com/library/VC0057/","api_url":"https://gliostate.com/api/items/VC0057.json"},{"id":"VC0059","title":"An Integrative Model of Cellular States, Plasticity, and Genetics for Glioblastoma.","year":"2019","page_url":"https://gliostate.com/library/VC0059/","api_url":"https://gliostate.com/api/items/VC0059.json"},{"id":"VC0063","title":"Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq.","year":"2017","page_url":"https://gliostate.com/library/VC0063/","api_url":"https://gliostate.com/api/items/VC0063.json"},{"id":"VC0064","title":"Developmental and oncogenic programs in H3K27M gliomas dissected by single-cell RNA-seq.","year":"2018","page_url":"https://gliostate.com/library/VC0064/","api_url":"https://gliostate.com/api/items/VC0064.json"},{"id":"VC0065","title":"The landscape of tumor cell states and spatial organization in H3-K27M mutant diffuse midline glioma across age and location.","year":"2022","page_url":"https://gliostate.com/library/VC0065/","api_url":"https://gliostate.com/api/items/VC0065.json"},{"id":"VC0068","title":"Single-cell RNA-seq reveals that glioblastoma recapitulates a normal neurodevelopmental hierarchy.","year":"2020","page_url":"https://gliostate.com/library/VC0068/","api_url":"https://gliostate.com/api/items/VC0068.json"},{"id":"VC0072","title":"A method for in silico exploration of potential glioblastoma multiforme attractors using single-cell RNA sequencing.","year":"2024","page_url":"https://gliostate.com/library/VC0072/","api_url":"https://gliostate.com/api/items/VC0072.json"},{"id":"VC0079","title":"Resolving medulloblastoma cellular architecture by single-cell genomics.","year":"2019","page_url":"https://gliostate.com/library/VC0079/","api_url":"https://gliostate.com/api/items/VC0079.json"},{"id":"VC0080","title":"Single-Cell Transcriptomics in Medulloblastoma Reveals Tumor-Initiating Progenitors and Oncogenic Cascades during Tumorigenesis and Relapse.","year":"2019","page_url":"https://gliostate.com/library/VC0080/","api_url":"https://gliostate.com/api/items/VC0080.json"},{"id":"VC0082","title":"Single-cell analysis reveals a longitudinal trajectory of meningioma evolution and heterogeneity.","year":"2025","page_url":"https://gliostate.com/library/VC0082/","api_url":"https://gliostate.com/api/items/VC0082.json"},{"id":"VC0083","title":"Single-cell profiling of human dura and meningioma reveals cellular meningeal landscape and insights into meningioma immune response.","year":"2022","page_url":"https://gliostate.com/library/VC0083/","api_url":"https://gliostate.com/api/items/VC0083.json"},{"id":"VC0087","title":"Single-cell transcriptomic analyses provide insights into the cellular origins and drivers of brain metastasis from lung adenocarcinoma.","year":"2023","page_url":"https://gliostate.com/library/VC0087/","api_url":"https://gliostate.com/api/items/VC0087.json"},{"id":"VC0132","title":"Drug and single-cell gene expression integration identifies sensitive and resistant glioblastoma cell populations","year":"2026","page_url":"https://gliostate.com/library/VC0132/","api_url":"https://gliostate.com/api/items/VC0132.json"}],"mentioned_in_topics":[{"id":"cell-state-mes-like","title_en":"Mesenchymal-like (MES-like) glioblastoma cell state","title_zh":"胶质母细胞瘤间充质样（MES 样）细胞状态","url":"https://gliostate.com/cell-states/mesenchymal-like/","api_url":"https://gliostate.com/api/topics/cell-state-mes-like.json"},{"id":"dataset-gbm-single-cell-spatial","title_en":"Glioblastoma single-cell and spatial datasets","title_zh":"胶质母细胞瘤单细胞与空间数据集","url":"https://gliostate.com/datasets/gbm-single-cell-spatial/","api_url":"https://gliostate.com/api/topics/dataset-gbm-single-cell-spatial.json"}],"relations":[],"related_entities":[{"id":"glioblastoma","type":"disease","name_en":"Glioblastoma (GBM)","name_zh":"胶质母细胞瘤（GBM）","page_url":"https://gliostate.com/entities/glioblastoma/","page_url_zh":"https://gliostate.com/zh/entities/glioblastoma/","api_url":"https://gliostate.com/api/entities/glioblastoma.json","via":"co-mention"},{"id":"neftel-four-state-framework","type":"method","name_en":"Neftel four-state framework","name_zh":"Neftel 四状态框架","page_url":"https://gliostate.com/entities/neftel-four-state-framework/","page_url_zh":"https://gliostate.com/zh/entities/neftel-four-state-framework/","api_url":"https://gliostate.com/api/entities/neftel-four-state-framework.json","via":"co-mention"},{"id":"gbmap","type":"dataset","name_en":"GBmap","name_zh":"GBmap","page_url":"https://gliostate.com/entities/gbmap/","page_url_zh":"https://gliostate.com/zh/entities/gbmap/","api_url":"https://gliostate.com/api/entities/gbmap.json","via":"co-mention"},{"id":"hypoxia","type":"pathway","name_en":"Hypoxia","name_zh":"缺氧","page_url":"https://gliostate.com/entities/hypoxia/","page_url_zh":"https://gliostate.com/zh/entities/hypoxia/","api_url":"https://gliostate.com/api/entities/hypoxia.json","via":"co-mention"},{"id":"opc-like-state","type":"cell_state","name_en":"Oligodendrocyte-progenitor-like (OPC-like) state","name_zh":"少突胶质前体细胞样（OPC 样）状态","page_url":"https://gliostate.com/entities/opc-like-state/","page_url_zh":"https://gliostate.com/zh/entities/opc-like-state/","api_url":"https://gliostate.com/api/entities/opc-like-state.json","via":"co-mention"},{"id":"ac-like-state","type":"cell_state","name_en":"Astrocyte-like (AC-like) state","name_zh":"星形胶质细胞样（AC 样）状态","page_url":"https://gliostate.com/entities/ac-like-state/","page_url_zh":"https://gliostate.com/zh/entities/ac-like-state/","api_url":"https://gliostate.com/api/entities/ac-like-state.json","via":"co-mention"},{"id":"lincs-l1000","type":"dataset","name_en":"LINCS L1000 drug signatures","name_zh":"LINCS L1000 药物特征谱","page_url":"https://gliostate.com/entities/lincs-l1000/","page_url_zh":"https://gliostate.com/zh/entities/lincs-l1000/","api_url":"https://gliostate.com/api/entities/lincs-l1000.json","via":"co-mention"},{"id":"mes-like-state","type":"cell_state","name_en":"Mesenchymal-like (MES-like) state","name_zh":"间充质样（MES 样）状态","page_url":"https://gliostate.com/entities/mes-like-state/","page_url_zh":"https://gliostate.com/zh/entities/mes-like-state/","api_url":"https://gliostate.com/api/entities/mes-like-state.json","via":"co-mention"}]}